コラーゲン技術研修会

since 1980

Ⅱ型コラーゲン(各種由来)

世界最初のⅡ型コラーゲン製品として30年の国内外使用実績。関節炎モデル、抗炎症剤のスクリーニングに、自己抗体や経口免疫寛容の研究に。研究用の各種由来のⅡ型コラーゲン(ウシ、ニワトリ、ブタ、サル、ヒト)を用意してます。
K41 タイプⅡコラーゲン液(K41 タイプⅡ液)

容液量: 8 ml(防腐剤無し)      

コラーゲン濃度: 0.3 %(3mg/ml)タイプⅡコラーゲン

形状: 溶液 (凍結)

保存: 短期-20 ℃ 長期-40 ℃以下 

原料: 牛関節軟骨

抽出: 酸・ペプシン処理

精製: 独自精製

K42 タイプⅡコラーゲン末(K42 タイプⅡ末)

含量: 24 mg/v      

形状: 凍結乾燥品

保存: 短期-20 ℃ 長期-40 ℃以下 

原料: ウシ関節軟骨

抽出: 酸・ペプシン処理

精製: 独自精製

K43 ニワトリタイプⅡコラーゲン末

含量: 10 mg       

形状: 凍結乾燥品

保存: 短期-20 ℃ 長期-40 ℃以下

原料: ニワトリ胸部軟骨

抽出: 酸・ペプシン処理

精製: 独自精製

※1.リウマチ関節炎モデルの惹起原。

※2.1981 年に世界で最初の製品化。

関連製品

・「K43」タイプⅡコラーゲン(ニワトリ由来)、他各種由来有り(ブタ、サル、ヒト、ラット)

・「K72 抗タイプⅡコラーゲン抗体測定システムELISA(ラット用)」

 「K71」ヒト用、「K73」マウス用

Ⅱ型コラーゲン使用論文

Bovine:

Nemoto N., et al. (2019) Analysis of intestinal immunity and flora in a collagen-induced mouse arthritis model: differences during arthritis progression.Int Immunol. 2019 Sep 28. pii: dxz058. 

Tomimori Y., et al. (2019) ASB17061, a novel chymase inhibitor, prevented the development of angiotensin II-induced abdominal aortic aneurysm in apolipoprotein E-deficient mice. Eur J Pharmacol. 2019 Aug 5;856:172403.

Hoshino N., et al. (2019) Role of Anti-Fractalkine Antibody in Suppression of Joint Destruction by Inhibiting Migration of Osteoclast Precursors to the Synovium in Experimental Arthritis.Arthritis Rheumatol. 2019 Feb;71(2):222-231.

Tsubosaka Y., et al. (2019) Hematopoietic prostaglandin D synthase-derived prostaglandin D2 ameliorates adjuvant-induced joint inflammation in mice. FASEB J. 2019 Jun;33(6):6829-6837.

Hiroyuki Y., et al. (2019) Effects of the Cathepsin K Inhibitor ONO-5334 and Concomitant Use of ONO-5334 with Methotrexate on Collagen-Induced Arthritis in Cynomolgus Monkeys. International Journal of Rheumatology Volume 2019, Article ID 5710340, 9 pages

Mohammad A., et al. (2019) Self-organization of hepatocyte morphogenesis depending on the size of collagen microbeads relative to hepatocytes. Biofabrication, Volume 11, Number 3

Minoru F., et al. (2015) Leucine-rich α2 -glycoprotein as a potential biomarker for joint inflammation during anti-interleukin-6 biologic therapy in rheumatoid arthritis. Arthritis & Rheumatology 67(8):2056-2060.

 

Hirota M., et al. (2016) Chemically Modified Interleukin-6 Aptamer InhibitsDevelopment of Collagen-Induced Arthritis in Cynomolgus Monkeys. Nucleic Acid Therapeutics 26(1):10-19.

Totsuka R., et al. (2016) Effects of VLA-1 Blockade on Experimental Inflammation in Mice. Kobe J Med Sci. 5;62(2):E27-37.

 

Sekine C., et al. (2014) Macrophage-derived delta-like protein 1 enhances interleukin-6 and matrix metalloproteinase 3 production by fibroblast-like synoviocytes in mice with collagen-induced arthritis. Arthritis Rheumatol. 66(10):2751-2761.

 

Uotsu Nobuo, et al. (2016) Suppression of Arthritis Progression with Lutein Extracted from Tagetes erecta in Collagen-Induced Arthritis Model Rats. Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry 16 (8):134-141.

 

Ito H., et al. (2016) Prokineticin 2 antagonist, PKRA7 suppresses arthritis in mice with collagen-induced arthritis. BMC Musculoskelet Disord. 17:387.

 

ShingoSugahara, et al. (2018) Integrin, alpha9 subunit blockade suppresses collagen-induced arthritis with minimal systemic immunomodulation. European Journal of Pharmacology 833: 320-327.

 

Kato G., et al. (2015) The inhibitory effects of a RANKL-binding peptide on articular and periarticular bone loss in a murine model of collagen-induced arthritis: a bone histomorphometric study. Arthritis Res Ther. 17(1): 251.

 

Yokoyama W., et al. (2014) Abrogation of CC chemokine receptor 9 ameliorates collagen-induced arthritis of mice. Arthritis Res Ther. 16(5):445.

 

Tanimoto A., et al. (2015) Pharmacological properties of JTE-052: a novel potent JAK inhibitor that suppresses various inflammatory responses in vitro and in vivo. Inflamm Res. 64(1):41-51.

 

Tanaka M., et al. (2016) Joint Degradation in a Monkey Model of Collagen-Induced Arthritis: Role of Cathepsin K Based on Biochemical Markers and Histological Evaluation. Int J Rheumatol. 8938916.

 

Otsuki N., et al. (2015) Modulation of immunological responses and amelioration of collagen-induced arthritis by the novel roxithromycin derivative 5-I. Mod Rheumatol. 25(4):562-70.

 

Yoshida H., et al. (2018) Anti-interleukin-6 receptor antibody prevents loss of bone structure and bone strength in collagen-induced arthritis mice. Scand J Rheumatol. 47(5):384-391.

 

Mizuno M., et al. (2017) The dual role of short fatty acid chains in the pathogenesis of autoimmune disease models. PLoS One. 12(2):e0173032.

 

Iizuka M., et al. (2014) Suppression of collagen-induced arthritis by oral administration of transgenic rice seeds expressing altered peptide ligands of type II collagen. Plant Biotechnol J. 12(8):1143-52.

 

Suzuki M., et al. (2015) Blockade of interleukin-6 receptor enhances the anti-arthritic effect of glucocorticoids without decreasing bone mineral density in mice with collagen-induced arthritis. Clin Exp Immunol. 182(2):154-61.

 

Hoshino-Negishi K., et al. (2018) Anti-Fractalkine Antibody Suppresses Joint Destruction by Inhibiting Migration of Osteoclast Precursors to the Synovium in Experimental Arthritis. Arthritis Rheumatol. doi: 10.1002/art.40688.

 

Takahashi Y., et al. (2014) Prophylactic and therapeutic effects of Acanthopanax senticosus Harms extract on murine collagen-induced arthritis. Phytother Res. 28(10):1513-9.

 

Fukuda S., et al. (2014) Cannabinoid receptor 2 as a potential therapeutic target in rheumatoid arthritis. BMC Musculoskelet Disord. 15:275.

 

Tanaka K., et al. (2014) Anti-interleukin-6 receptor antibody prevents systemic bone mass loss via reducing the number of osteoclast precursors in bone marrow in a collagen-induced arthritis model. Clin Exp Immunol. 175(2):172-80.

 

Murakoshi M., et al. (2017) Discovery and pharmacological effects of a novel GPR142 antagonist. J Recept Signal Transduct Res. 37(3):290-296.

 

Gao S., et al. (2017) Total sesquiterpene lactones prepared from Inula helenium L. has potentials in prevention and therapy of rheumatoid arthritis. J Ethnopharmacol. 196:39-46.

 

Iizuka M., et al. (2014) Prophylactic effect of the oral administration of transgenic rice seeds containing altered peptide ligands of type II collagen on rheumatoid arthritis. Biosci Biotechnol Biochem. 78(10):1662-8.

 

Tatsuya Tamura, et al. (2016) Novel hyaluronic acid–methotrexate conjugate suppresses joint inflammation in the rat knee: efficacy and safety evaluation in two rat arthritis models. Arthritis Res Ther. 18: 121.

 

Murata Y., et al. (2014) Autoimmune animal models in the analysis of the CD47-SIRPα signaling pathway. Methods. 65(2):254-9.

 

Oike T., et al. (2017) Stat3 as a potential therapeutic target for rheumatoid arthritis. Sci Rep. 7(1):10965.

chicken:

Koji Sakuraba, et al. (2016) Interleukin-21 signaling in B cells, but not in T cells, is indispensable for the development of collagen-induced arthritis in mice. Arthritis Research & Therapy 18:188.

Type Ⅱ Collagen:

Kumai T., et al. (2019) A novel, self-assembled artificial cartilage-hydroxyapatite conjugate for combined articular cartilage and subchondral bone repair: histopathological analysis of cartilage tissue engineering in rat knee joints. Int J Nanomedicine. 2019 Feb 19;14:1283-1298.

Kanagawa H., et al. (2015) Mycobacterium tuberculosis promotes arthritis development through Toll-like receptor 2. J Bone Miner Metab. 33(2):135-41.

 

Shimomura S., et al. (2018) Treadmill Running Ameliorates Destruction of Articular Cartilage and Subchondral Bone, Not Only Synovitis, in a Rheumatoid Arthritis Rat Model. Int J Mol Sci. 19(6). pii: E1653.

 

Kato T., et al. (2017) JTE-852, a novel spleen tyrosine kinase inhibitor, blocks immunoglobulin G-mediated cellular responses and autoimmune reactions in vivo. Life Sci. 191:166-174.

 

Gao J., et al. (2015) A disintegrin and metallproteinase 15 knockout decreases migration of fibroblast-like synoviocytes and inflammation in rheumatoid arthritis. Mol Med Rep. 11(6):4389-96.

 

Inoue H., et al. (2014) Sonoporation-mediated transduction of siRNA ameliorated experimental arthritis using 3 MHz pulsed ultrasound. Ultrasonics. 54(3):874-81.

 

1)山津功,千葉健一. Ⅱ型コラーゲン関節炎. In:大河内和雄 編
  炎症とアレルギー 東京:広川書店 1993: 163-175

2)柿本穀一, 小俣節子. アジュバンド, コラーゲン関節炎の誘導.
  In: 高津聖志, 瀧伸介 編 免疫研究の基礎知識 東京:羊土社
  1995: 283-290.

3) F.Kato,et al.:Annals of the Rheumatic Diseases 1996;55:535-539.